Improving the molecular diagnosis of Chlamydia psittaci and Chlamydia abortus infection with a species-specific duplex real-time PCR
نویسندگان
چکیده
منابع مشابه
Development of TaqMan Duplex Real-time PCR for Simultaneous Detection of Chlamydia Trachomatis and Mycoplasma Genitalium
Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
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A patient presenting with subacute thyroiditis who had evidence of chlamydial infection is reported.
متن کاملDevelopment of a Chlamydophila psittaci species-specific and genotype-specific real-time PCR.
A Chlamydophila psittaci species-specific real-time PCR targeting the rDNA ribosomal spacer was developed as well as a genotype-specific real-time PCR targeting the Cp. psittaci outer membrane protein A (ompA) gene. The SYBR Green-based species-specific real-time PCR detected Cp. psittaci genotypes A to F, and the recently discovered E/B genotype. The genotype-specific real-time PCR could easil...
متن کاملDetection of Chlamydia pneumoniae and Chlamydia psittaci in sputum samples by PCR.
AIMS To use the polymerase chain reaction (PCR) to detect Chlamydia pneumoniae and Chlamydia psittaci in sputum samples. METHODS A nested PCR was developed, the first stage of which amplified DNA from both C pneumoniae and C psittaci while the second stage targeted specifically at C pneumoniae, allowing the two species to be differentiated. The primers were designed not to amplify sequences f...
متن کاملSeroprevalence of Chlamydia abortus infection in sheep and goats in Iran
BACKGROUND: Chlamydia abortus is a zoonotic bacteriumthat commonly causes abortions in ruminants. This microorganismis one of the most important infectious agents causingabortion and major economic losses in sheep and goats worldwide.OBJECTIVES: The objective of this study was investigatingthe status of C. abortus infection among small ruminantflocks of some regions in Iran. METHODS: A total of...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2015
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.000139